Corbett rotor gene 60003/13/2024 click on “Edit Profile” to change run parameters section to be altered is greyed out, click on “Hold Temperature” to change temperature, click on “Hold Time” to change time Always read manufacturer’s instructions regarding Hot start Taq activation timesĬlick on “Cycling” to adjust the cycling parameters Default cycle number is 40 To change a step click on the temperature and adjust then click on the time and adjustĭata is acquired each cycle (blue dots) No blue dots = no data = very bad Acquire at the end of the extension phase Click on “Aquiring to Cycling A” to select colours Move the colours you want from “Available Channels” to “Acquiring Channels” using arrows Use Green channel for SYBR green Insert user name, any notes (primer conc, primer sequence mix used etc) and volume required (10-25uL) This info is saved and can be printed in reports Click “Next” Select the rotor you are using, put the rotor into the machine with the locking ring attached The locking ring tick box must be ticked to proceed Click “Next” Getting Started…… Click on Rotor-Gene icon Click on advanced tab for step by step run set up Choose run template depending on chemistry Dual labeled probes = hydrolysis probes SYBR = intercalating dyes The world’s only real-time rotary thermo-optical analyser ![]() ![]() Corbett LIFE SCIENCE Getting Started on the Rotor-Gene-6000 Jennifer McMahon, PhD
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